The performance and identification of Lung cell tissue on formalin fixed animal material to proceed to the immunochistochemistry and microscopy applications
Results of approached DNA extraction protocol; the genomic DNA from Strawberry, Banana, Kiwi
Results of fruits homogenizing follow the DNA extraction protocol; the homogenates been isolate from Strawberry, Banana, Kiwi
During this laboratory students used agarose gel electrophoresis to separate DNA fragments. While the gel is cooling, prepare the DNA samples by adding 1 uL of tracking marker mixed with loading buffer
Practical experience by Raymond
Continue loading the samples and finish of with a final lane of marker
The gel loaded into the chamber with the gel oriented such that the wells are cover by TBE running buffer, DNA will migrate away from negative(left) to positive (positive) side.
Close the gel tank ,switch on the power-source and run the gel at 5 Vcm. The electrodes are 10 cm apart running condition are 50 V.
Monitor the progress of the gel by reference to the marker dye. Stop the gel when the bromophenol blue has run 3/4 the length of the gel.
Used sterile technique, pour 12-15 ml of LB-agar into a Petri dish (enough to cover the bottom of the dish) Transformed DNA plated into LB with Kanamycin overnight expression.
Students attending presentation given by Assoc/Prof Paul Rigby during the guide tour to Centre for Microscopy, Characterisation and Analysis at University of Western Australia (UWA). He demonstrated and performed for PCNM students, a selection of the modern microscopy applications and microanalysis facilities, include Nuclear magnetic resonance (NMR) spectroscopy, mass spectrometer, assorted multifocal and confocal microscopes, cell sorters instruments.
The discussion label research excellence by providing world class results and expertise to publicly funded researchers and industry. The data management in relation to the analysis and visualization, adopted visualization software to images creation been performed within discussion to student’s observation